Our group has demonstrated expertise through various publications in working with samples of diverse origins at multiple molecular levels, spanning from genes to proteins. We have employed a variety of technical approaches to accomplish this:

DNA:
– Epigenetics: gene metilation
– Gene sequencing

RNA:
– Gene expression: RT-PCR, in situ hybridization
– Gene expression regulation: non-coding RNA

Protein:
– Distribution: immunohistochemistry, immunofluorescence
– Quantification: western blotting, ELISA, dot blot
– SIMOA (SIngle MOlecule Array): CSF, serum, plasma or tissue

Enzyme activity assay and flux cytometry

Omics: Transcriptomics (microarrays, RNA-seq), proteomics (Orbitrap, redox proteomics, phospho-proteomics), metabolomics and lipidomics